Assay particles and methods of use

摘要

The invention provides assay particles useful, for example, for detecting analytes and binding molecule interactions. One type of assay particle includes a core portion encased by a shell portion, wherein the shell portion comprises an inorganic phosphor that binds selectively to a target molecule. Another type of an assay particle includes a core portion encased by a shell portion, and a coat portion covering the shell portion, wherein the coat portion comprises an inorganic phosphor that binds selectively to a target molecule. A further type of assay particle includes a core portion encased by a shell portion, and a coat portion covering the shell portion, wherein the coat portion comprises an inorganic phosphor and a target selective binding moiety, and wherein the assay particle is buoyant in aqueous media. An additional type of assay particle includes a core portion encased by a shell portion, and a coat portion covering the shell portion, wherein the shell portion comprises an inorganic phosphor and the coat portion comprises a target selective binding moiety, and wherein the assay particle is buoyant in aqueous media. Also provided are kits and related methods.

主权项

1. A method for detecting protein kinase activity, the method comprising:
contacting a sample suspected of containing a protein kinase with: (i) an assay particle, wherein the assay particle comprises:
a core portion encased by a shell portion; anda coat portion covering the shell portion,wherein the shell portion of the assay particle comprises titanium dioxide and a material selected from the group consisting of glass, ceramic, and combinations thereof;wherein the coat portion comprises titanium dioxide and a target selective binding agent; andwherein the assay particle is hollow and is buoyant in aqueous media; and (ii) a protein kinase substrate comprising a moiety capable of emitting radiation under conditions wherein the protein kinase can phosphorylate the substrate to produce a phosphorylated substrate; wherein binding of the phosphorylated substrate to titanium dioxide produces a light signal; and determining a protein kinase activity based on a level of detected light signal.