Methods of preventing and removing trisulfide bonds

摘要

The present invention pertains to methods of preventing and eliminating trisulfide bonds in proteins such as antibodies. In one embodiment, trisulfide bonds in proteins are converted to disulfide bonds as part of chromatographic purification procedures. In another embodiment, the formation of trisulfide bonds in proteins is inhibited by implementation of methods described herein during the cell culture production of such proteins. In another embodiment, monoclonal antibodies are produced by the methods described herein.

主权项

1. A method for reducing the formation of trisulfide bonds in a protein comprising culturing cells expressing said protein in the presence of an effective amount of an inhibitor of cysteine degradation, whereby trisulfide linkage formation in said protein is reduced relative to cells cultured in medium without the inhibitor of cysteine degradation, and wherein said inhibitor is methyl pyruvate, ethyl pyruvate, glyceraldehyde, or glyoxylic acid.