| 摘要 |
The invention provides a non-naturally occurring microbial organism having a muconate pathway having at least one exogenous nucleic acid encoding a muconate pathway enzyme expressed in a sufficient amount to produce muconate. The muconate pathway including an enzyme selected from the group consisting of a beta-ketothiolase, a beta-ketoadipyl-CoA hydrolase, a beta-ketoadipyl-CoA transferase, a beta-ketoadipyl-CoA ligase, a 2-fumarylacetate reductase, a 2-fumarylacetate dehydrogenase, a trans-3-hydroxy-4-hexendioate dehydratase, a 2-fumarylacetate aminotransferase, a 2-fumarylacetate aminating oxidoreductase, a trans-3-amino-4-hexenoate deaminase, a beta-ketoadipate enol-lactone hydrolase, a muconolactone isomerase, a muconate cycloisomerase, a beta-ketoadipyl-CoA dehydrogenase, a 3-hydroxyadipyl-CoA dehydratase, a 2,3-dehydroadipyl-CoA transferase, a 2,3-dehydroadipyl-CoA hydrolase, a 2,3-dehydroadipyl-CoA ligase, a muconate reductase, a 2-maleylacetate reductase, a 2-maleylacetate dehydrogenase, a cis-3-hydroxy-4-hexendioate dehydratase, a 2-maleylacetate aminoatransferase, a 2-maleylacetate aminating oxidoreductase, a cis-3-amino-4-hexendioate deaminase, and a muconate cis/trans isomerase. Other muconate pathway enzymes also are provided. Additionally provided are methods of producing muconate. |
| 主权项 |
1. A transformed Escherichia coli having a muconate pathway that produces muconate pathway enzymes, said muconate pathway enzymes comprising a (1) beta-ketothiolase, (2) an enzyme selected from beta-ketoadipyl-CoA hydrolase and beta-ketoadipyl-CoA transferase, (3) beta-ketoadipate enol-lactone hydrolase, (4) muconolactone isomerase, (5) muconate cycloisomerase, and (6) muconate cis/trans isomerase, wherein said Escherichia coli comprises at least one exogenous nucleic acid encoding a muconate pathway enzyme obtained from bacteria, yeast, fungi, plant or mammal, and wherein the at least one exogenous nucleic acid is overexpressed resulting in increased synthesis or accumulation of a muconate. |
