Method for the production of duplication and translocation of any region in the chromosome of aspergillus

摘要

The purpose of the present invention is to provide a technique to duplicate and translocate any large region in a chromosome of a fungus belonging to Aspergillus across a wide range, so that it will possible to stably and systematically acquire an Aspergillus strain having a novel trait, which was un-acquirable by conventional techniques.;The present invention relates to a transformant of the fungus belonging to Aspergillus wherein a transformation marker gene with deficiency of a terminal part at the 5′ or 3′ end of its coding region is integrated into an outside of a target region in a chromosome of the fungus subject to duplicated translocation, and a transformation marker gene with deficiency of a terminal part at the 3′ or 5′ end of its coding region is integrated into an outside of a region in another chromosome of the fungus to be replaced with the target region.

主权项

1. A transformant of a fungus belonging to Aspergillus, comprising,
a first transformation marker gene fragment with a 3′ terminal deficiency integrated in a reverse direction into the 5′ end of a target region capable of duplicated translocation wherein the target region is located in a long arm of a first chromosome, or said marker gene fragment is integrated in a forward direction into the 3′ end of a target region capable of duplicated translocation wherein the target region is located in a short arm of the first chromosome; and a second transformation marker gene fragment, which is the same as the first transformation marker gene fragment, except that it has a 5′ terminal deficiency, integrated in a reverse direction into the 5′ end of a region to be replaced with one of said target regions wherein the region to be replaced is located in a long arm of a second chromosome, or said marker gene fragment is integrated in a forward direction into the 3′ end of a region to be replaced with one of said target regions wherein the region to be replaced is located in a short arm of the second chromosome; wherein a homologous sequence region is present in common in a middle portion of a coding region of both of the transformation marker gene fragments.