Organic colored microparticles, diagnostic reagent kit containing the same, and in vitro diagnosis method

摘要

Provided are an immunochromatography kit that is highly sensitive and capable of multicoloration, and organic colored microparticles that are ideal as an element of the immunochromatography kit. Organic colored microparticles having an average grain size between 10 and 1,000 nm and a color intensity between 1.0 and 5.0 are prepared using cellulose as the starting material. When the organic colored microparticles are used as a label in an immunochromatography kit, the immunochromatography kit is of a high sensitivity than conventional technology. The immunochromatography kit is also capable of multicoloration and is useful for rapid diagnosis.

主权项

1. Colored cellulose microparticles for use in an immunochromatography kit,
wherein the colored cellulose microparticles have an average grain size of 10 nm to 1000 nm and a color intensity of 1.0 to 5.0, wherein 10% by weight to 80% by weight of the colored cellulose microparticles constitute a coloring component which is a reactive dye, and said color intensity being obtained by:
preparing a dispersion consisting of the colored cellulose microparticles in water as a dispersion medium at a concentration ranging from 0.01% to 0.1% by weight,measuring the optical absorbance of said dispersion between 400 and 800 nm using a combination of the SV-722 Integrating Sphere and the JASCO-V-650 manufactured by Jasco Corp and an optical path of 10 mm, thereby obtaining a sample absorbance curve,measuring the optical absorbance of the dispersion medium between 400 and 800 nm using said integrating sphere and an optical path of 10 mm, thereby obtaining a background absorbance curve,subtracting the background absorbance curve from the sample absorbance curve, thereby obtaining a corrected absorbance curve, said corrected absorbance curve having a peak with a maximum absorbance value, anddividing the maximum absorbance value of the peak of the corrected absorbance curve by the concentration in % by weight of the dispersion, and then standardized per 0.01% by weight, thereby obtaining the color intensity.