发明名称 |
Method for preparing eptifibatide |
摘要 |
Provided is a method for preparing eptifibatide, wherein the method comprises: obtaining an eptifibatide refined peptide solution; and obtaining and freeze-drying an eptifibatide refined peptide concentrate after rotary evaporation of the eptifibatide refined peptide solution, wherein the concentration of the eptifibatide refined peptide concentrate is 15-30 mg/ml and the temperature of rotary evaporation is 251° C.-35° C. The preparation method of the eptifibatide refined peptide solution is as follows: coupling Cys with a resin to obtain a Cys-resin; obtaining a polypeptide having a sequence as represented by SEQ ID NO: 1 by gradually coupling the Cys-resin with Pro, Trp, Asp, Gly, Har and Mpa; and obtaining the eptifibatide refined peptide solution through oxidation, cleavage, purification and transfer to salt. |
申请公布号 |
US9562071(B2) |
申请公布日期 |
2017.02.07 |
申请号 |
US201414392203 |
申请日期 |
2014.06.16 |
申请人 |
HYBIO PHARMACEUTICAL CO., LTD. |
发明人 |
Kang Xu;Liu Jian;Ma Yaping;Yuan Jiancheng |
分类号 |
A61K38/04;C07K5/00;C07K7/00;C07K16/00;C07K17/00;C07K7/06 |
主分类号 |
A61K38/04 |
代理机构 |
Parker Highlander PLLC |
代理人 |
Parker Highlander PLLC |
主权项 |
1. A method for preparing eptifibatide, characterized in comprising:
(a) obtaining a refined eptifibatide peptide solution; (b) rotarily evaporating the refined eptifibatide peptide solution, obtaining and freeze-drying a refined eptifibatide peptide concentrate; wherein the concentration of the refined eptifibatide peptide concentrate is 15-20 mg/mL; wherein the temperature of the rotary evaporation is 30° C.-35° C.; and wherein the refined eptifibatide peptide solution is prepared by obtaining a Cys-resin by coupling Cys to a resin; obtaining a polypeptide having a sequence as represented by SEQ ID NO: 1 by sequentially coupling the Cys-resin with Pro, Trp, Asp, Gly, Har and Mpa; and obtaining the refined eptifibatide peptide solution through oxidation, cleavage, purification and salt conversion. |
地址 |
Shenzhen CN |