Device for isolating an analyte from a sample, and methods of use

摘要

A device for extraction or isolation of an analyte, such as a nucleic acid, a protein, or a cell, from a sample, and in particular from a biological sample is described. Methods of using the device are also described.

主权项

1. A device, comprising:
a rigid body having a first face and a second face, and defining within said first face: a first cavity having an outlet, a second cavity, and a third cavity, a first flow path positioned between and connecting the outlet of the first cavity and the second cavity, said outlet comprising a constricted region between said first cavity and said first flow path, wherein the outlet constricted region is more narrow than the first flow path, and a second flow path positioned between and connecting the second cavity and the third cavity, the second flow path comprising an upper and a lower surface and a barrier region having a top surface which is above the level of the lower surface of the second flow path; wherein the first flow path is contiguous with the second flow path; a wall member secured to at least a portion of the first face of the rigid body, said wall member disposed over the first cavity, the second cavity, and the third cavity, thereby defining a first chamber, a second chamber, and a third chamber, the third chamber having a width and a length, wherein the length is greater than the width; and one or more inlet ports in direct communication with at least the first chamber and the third chamber for introduction of one or more water-miscible liquid reagents into each of the first and third chambers.