Method of detecting OXA-048 carbapenemase producing bacteria

摘要

A method of detecting and/or specifically identifying bacteria producing OXA-048-type carbapenemase in a biological sample, includes the steps of: (a) placing the biological sample likely to contain the bacteria in contact with a reaction medium including at least one chromogenic substrate to detect an enzymatic activity, and temocillin at a concentration equal to or greater than 150 mg/L, preferably between 200 and 500 mg/L, (b) incubating the sample in the medium to allow the bacteria to grow, and (c) detecting the strains corresponding to the OXA-48 carbapenemase producing bacteria. A culture medium as implemented in step (a) is also provided.

主权项

1. A method for specifically distinguishing OXA-48 carbapenemase-producing bacteria from other types of carbapenem-resistant bacteria in a biological sample, comprising:
contacting the biological sample with a reaction medium that comprises temocillin at a concentration greater than or equal to 150 mg/l and at least one chromogenic substrate; incubating the reaction medium contacted with the biological sample so as to allow growth of OXA-48 carbapenemase-producing bacteria if present; and specifically detecting whether there is growth of OXA-48 carbapenemase-producing bacteria, to the exclusion of other types of carbapenem-resistant bacteria, on or in the reaction medium in order to discriminate OXA-carbapenemase-producing bacteria from other types of carbapenem-resistant bacteria, wherein the biological sample comprises carbapenem-resistant bacteria.