Biosensor with improved interference characteristics

摘要

This invention relates to mutagenized redox oxidase enzymes used to design enzyme electrodes with improved interference characteristics in the presence of mediator and oxygen in the assay. This recombinant modified enzyme has enhanced capability to transfer electrons to redox mediator instead of its natural electron acceptors such as O2, NAD, NADP, etc., which will improve the assay performance with less interference and higher sensitivity.

主权项

1. A device for detecting a target analyte, the device comprising:
an electrochemical cell; an electron redox mediating molecule; and a recombinant modified enzyme engineered to:
prevent transfer of electrons to a natural electron acceptor;transfer electrons to the electron redox mediating molecule; andprovide enzymatic activity against the target analyte to generate an electrochemical signal dependent on a concentration of the target analyte in a sample.