METHODS AND COMPOSITIONS FOR SECRETION OF HETEROLOGOUS POLYPEPTIDES

摘要

The present invention relates generally to the fields of molecular biology and protein technology. More specifically, the invention concerns signal sequences for the secretion of heterologous polypeptide from bacteria. The invention also concerns recombinant polypeptides and uses thereof.

主权项

1. A method of increasing secretion of an antibody heavy chain and/or an antibody light chain from an E. coli host cell, comprising culturing an E. coli host cell comprising polynucleotide comprising (1) a first polynucleotide encoding a first signal peptide operably linked to a polynucleotide encoding an antibody heavy chain, wherein the average hydrophobicity of the signal peptide is greater than about 0.5; and/or (2) a second polynucleotide encoding a second signal peptide operably linked to a polynucleotide encoding an antibody light chain, wherein the average hydrophobicity of the second signal peptide is greater than about 0.5, whereby upon expression of the antibody in a host cell, the heavy and light chains are folded and assembled to form a biologically active antibody.