METHOD AND COMPOSITION USING A QUATERNARY AMMONIUM COMPOUND

摘要

A method of determining of the presence or absence of a target RNA component in a sample, the method comprising steps of: • (a) contacting the sample with a composition comprising • (i) a quaternary ammonium compound or a precursor thereof, which should extract / release the nucleic acid from the sample; • (b) optionally storing and/or transporting the composition obtained in step (a); • (c) contacting the composition obtained in step (a) with a composition comprising a proteinaceous washing agent, e.g. BSA or casein for stabilizing the RNA for transport and/or storage.; and • (d) using the composition obtained in step (c) in an amplification method, e.g. RT-PCR.