| 发明名称 |
PROTEIN PRODUCTION |
| 摘要 |
A method for producing from host cells a heterologous polypeptide or protein comprising at least one cysteine residue in a reduced state. The method cultured the host cells in a culture medium comprising a reducing agent; and recovered the heterologous polypeptide or protein comprising at least one cysteine residue in a reduced state from the host cells or from the culture medium, where the host cells comprise a nucleic acid encoding the heterologous protein or polypeptide, and where the reducing agent is capable of permeating the plasma membrane of the host cells. |
| 申请公布号 |
US2017029861(A1) |
申请公布日期 |
2017.02.02 |
| 申请号 |
US201615200079 |
申请日期 |
2016.07.01 |
| 申请人 |
Siurkus Juozas;Neubauer Peter |
发明人 |
Siurkus Juozas;Neubauer Peter |
| 分类号 |
C12P21/02;C12N1/20 |
主分类号 |
C12P21/02 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method for producing from host cells an active heterologous polypeptide or protein comprising at least one cysteine residue in a reduced state, the method comprising
(i) culturing host cells in a culture medium comprising a reducing agent having a reduction potential stronger than −0.1V at pH 7 and 25° C.; and (ii) recovering the heterologous polypeptide or protein comprising at least one cysteine residue in a reduced state from said host cells or from the culture medium,where the host cells comprise a nucleic acid encoding the heterologous protein or polypeptide, and where the reducing agent is capable of permeating the plasma membrane of the host cells. |
| 地址 |
Kaisiadorys LT |
