METHODS FOR SINGLE CHAIN VARIABLE REGION ENOX2 ANTIBODIES FOR CANCER DETECTION AND DIAGNOSIS

摘要

Cancers of different cellular or tissue origins express different ENOX2 cancer isoforms or combinations of isoforms and shed these proteins into the circulation. Herein are disclosed methods both for cancer detection and diagnosis of particular origin, based on the patterns and molecular weights of the isoforms which allow the identification of the cell type and or tissue of origin of the neoplasm. Relative ENOX2 amounts are proportional to tumor burden and provide a reliable measure of response to therapy and disease progression. Also provided is the amino acid sequence to which the scFv antibodies bind as the molecular basis for the specificity of the test.

主权项

1. A method for detecting the presence of one or more ENOX2 transcript variants comprising:
obtaining a sample suspected of having one or more ENOX2 transcript variants; electrophoretically separating the one or more ENOX2 transcript variants; and determining the presence of the one or more ENOX2 transcript variants using a single chain variable region ENOX2 antibody linked directly to an alkaline phosphatase or other single step agent for detection or imaging; and