METHODS AND COMPOSITIONS FOR MODIFYING GENOMIC DNA

摘要

Compositions and methods concern the sequence modification of an endogenous genomic DNA region. Certain aspects relate to a method for site-specific sequence modification of a target genomic DNA region in cells comprising: transfecting the cells by electroporation with a composition comprising (a) a DNA oligo and (b) a DNA digesting agent wherein the donor DNA comprises: (i) a homologous region comprising nucleic acid sequence homologous to the target genomic DNA region and (ii) a sequence modification region; and wherein the genomic DNA sequence is modified specifically at the target genomic DNA region.

主权项

1. A method for site-specific sequence modification of a target genomic DNA region in cells comprising:
transfecting the cells by electroporation with a composition comprising (a) a DNA oligo having 100 nucleotides or less and (b) a DNA digesting agent encoded on an RNA; wherein the DNA oligo comprises:
(i) a homologous region comprising DNA sequence homologous to the target genomic DNA region; and(ii) a sequence modification region; and wherein the genomic DNA sequence is modified specifically at the target genomic DNA region, and wherein the cells are stem cells or their progeny.