Scanning Imaging For Encoded PSF Identification and Light Field Imaging

摘要

Scanned illumination allows for capturing 3-dimensional information about an object. A conventional reflection (or transmission) bright field (or fluorescence, darkfield, polarizing, phase contrast or interference) microscope is configured to use laterally scanned illumination (for example by moving an array in front of the light source) to scan an extended object. A pixelated detector may capture a series of images at the exit pupil of a microscope objective, and this series of images may be processed to form a Light Field image of the object. Or, a microscope is configured to provide scanned illumination to an extended object, while applying extended depth of field and 3D depth localization encoding to the resulting set of images. Thus multiple encoded images are generated. These images are decoded and combined, with custom digital signal processing algorithms, to form a 3D volume rendering or animation. Each point in the specimen is illuminated separately from its neighbors, and records its distinct PSF signature without any ambiguity arising from adjacent points on the object.

主权项

1. Apparatus for capturing a Light Field of an object comprising:
a scanning device for sequentially illuminating points of the object; an optic for focusing light from the object; a detector having an array of pixels for forming a series of multi-pixel images at a focal plane near the exit pupil of the optic; a storage device for storing the series of multi-pixel images.