SPECTROSCOPIC METHODS FOR THE DETECTION OF GLYCATED HEMOGLOBIN

摘要

Kits, microfluidics devices, and assays for use in methods of spectroscopically determining a ratio of glycated hemoglobin to total hemoglobin in a whole blood sample are disclosed.

主权项

1. A kit for determining a ratio of glycated hemoglobin to total hemoglobin, the kit comprising:
a blood separation membrane, wherein the blood separation membrane traps red blood cells thereon and/or therein, and wherein plasma passes through the blood separation membrane; a waste collection material capable of being associated with the blood separation membrane and capable of wicking waste material away from the blood separation membrane and into the waste collection material; a negatively charged surface, wherein the negatively charged surface traps hemoglobin thereon and/or therein, and wherein other components of lysed red blood cells are not trapped on the negatively charged surface, and wherein the negatively charged surface is capable of being subjected to spectroscopy to measure an amount of total hemoglobin trapped thereon and/or therein and an amount of glycated hemoglobin or non-glycated hemoglobin trapped thereon and/or therein; and a waste collection material capable of being associated with the negatively charged surface and capable of wicking waste material away from the negatively charged surface and into the waste collection material.