FAST THERMO-OPTICAL PARTICLE CHARACTERISATION

摘要

The present invention relates to a method and an apparatus for a fast thermo-optical characterisation of particles. In particular, the present invention relates to a method and a device to measure the stability of (bio)molecules, the interaction of molecules, in particular biomolecules, with, e.g. further (bio)molecules, particularly modified (bio)molecules, particles, beads, and/or the determination of the length/size (e.g. hydrodynamic radius) of individual (bio)molecules, particles, beads and/or the determination of length/size (e.g. hydrodynamic radius).

主权项

1. Method to measure thermo-optically characteristics of particles in a solution with the steps of:
providing a sample probe with marked particles in a solution; exciting fluorescently said marked particles and firstly detecting fluorescence of said excited particles; irradiating a laser light beam into the solution to obtain a spatial temperature distribution in the solution around the irradiated laser light beam; detecting secondly a fluorescence of the particles in the solution at a predetermined time after irradiation of the laser into the solution has been started, and characterizing the particles based on said two detections.