METHODS AND SYSTEMS FOR DROPLET TAGGING AND AMPLIFICATION

摘要

The present invention generally relates to microfluidic devices, including methods and systems for tagging droplets within such devices. In some aspects, microfiuidic droplets are manipulated by exposing the droplets (or other discrete entities) to a variety of different conditions. By incorporating into the droplets a plurality of nucleic acid “tags,” and optionally amplifying the nucleic acids, e.g., within the droplets, the conditions that a droplet was exposed to may be encoded by the nucleic acids. Thus, even if droplets exposed to different conditions are mixed together, the conditions that each droplet encountered may still be determined, for example, by sequencing the nucleic acids.

主权项

1. A method, comprising:
exposing a microfluidic droplet to a first condition and adding a first nucleic acid to the droplet, wherein the first nucleic acid encodes the first condition; exposing the microfluidic droplet to a second condition and adding a second nucleic acid to the droplet, wherein the second nucleic acid encodes the second condition; and amplifying the first nucleic acid and the second nucleic acid within the microfluidic droplet.