METHODS AND DEVICES FOR NUCLEIC ACID PURIFICATION

摘要

The invention provides pipette tip columns and automated methods for the purification of nucleic acids including plasmids. Nucleic acids can be purified from unclarified, clarified or partially-clarified cell lysates that contain cell debris. The columns typically include a bed of medium positioned in the pipette tip column, above a bottom frit and with an optional top frit. Plasmid preparation scales include miniprep, midiprep, maxiprep, megaprep and gigaprep.

主权项

1. A method for capturing plasmid DNA on a column, comprised of:
a) providing a column, wherein the column is comprised of a solid phase; b) providing a cell lysate comprised of a liquid and a flocculent, wherein the liquid contains plasmid DNA; c) providing a large-pore filter having a pore size, wherein the pore size of the large-pore filter is greater than 50 μm; d) passing the cell lysate through the large-pore filter to produce a partially clarified lysate; and e) passing the partially clarified lysate over the column, wherein a portion of the plasmid DNA is captured on the solid phase.