发明名称 |
METHOD FOR FABRICATING CELL AGGREGATE FOR SELF-ORGANIZATION |
摘要 |
The present invention finds out find out the requirements necessary for preparing a cell condensate in vitro from a large number of cells (several ten thousand to several million cells) and provides a method of forming a cell condensate for self-organization which is capable of realizing complex higher structures (such as liver and kidney) and interactions with other organs. A method of preparing a cell condensate in vitro, comprising culturing a mixture of cells and/or tissues of a desired type in a total cell count of 400,000 or more and 100,000 to 400,000 mesenchymal cells to form a cell condensate of 1 mm or more in size. A cell condensate prepared by the above-described method. A method of preparing a three-dimensional tissue structure, comprising allowing self-organization of a cell condensate prepared by the above-described method to form a three-dimensional tissue structure that has been provided with higher structures. A gel-like support wherein the side on which culture is to be performed has a U- or V-shaped cross-section. |
申请公布号 |
EP3124600(A1) |
申请公布日期 |
2017.02.01 |
申请号 |
EP20150754683 |
申请日期 |
2015.02.26 |
申请人 |
Public University Corporation Yokohama City University;National University Corporation Saitama University |
发明人 |
TAKEBE, Takanori;TANIGUCHI, Hideki;YOSHIKAWA, Hiroshi |
分类号 |
C12N5/071;A61L27/00;C12M3/00;C12N5/0735;C12N5/077;C12N5/0775;C12N5/10 |
主分类号 |
C12N5/071 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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