Insect resistance management with combinations of Cry1Be and Cry1F proteins

摘要

The subject invention relates in part to stacking Cry1Be toxins along with Cry1Fa toxins to prevent insects from developing resistance towards either toxin by itself. As discussed in more detail herein, the subject pair of proteins is a particularly advantageous combination, as no other pair of proteins is known to provide high levels of control and non-cross-resistant activity against both Spodoptera frugiperda (FAW) and Ostrinia nubilalis (ECB) insects. This dual, non-cross-resistant activity is also advantageous because it can reduce the number of proteins/genes needed to target these insects with multiple, non-cross-resistant proteins. This can reduce or eliminate the need for refuge acreage. Accordingly, the subject invention also relates generally to using four genes to provide three proteins for non-cross-resistant control of a first insect, and three proteins for non-cross-resistant control of a second insect. In preferred embodiments, the targeted insects are FAW and ECB.

主权项

1. A transgenic plant comprising DNA encoding a Cry1Be insecticidal protein and DNA encoding a Cry1Fa insecticidal protein, wherein said Cry1Fa insecticidal protein is at least 95% identical with SEQ ID NO:1, and said Cry1Be insecticidal protein is at least 95% identical with SEQ ID NO:2, wherein said transgenic plant expresses an effective amount of said Cry1Fa insecticidal protein and said Cry1Be insecticidal protein to prevent development of Cry-resistant lepidopteran pests, and wherein said Cry1Fa insecticidal protein and said Cry1Be insecticidal protein do not share a receptor binding site in European corn borer (ECB) gut and do not share a receptor binding site in fall armyworm (FAW) gut.