Image processing device and storage medium for image processing

摘要

An image processing device inputs a bright field linage of a tissue slice in which a cell nucleus is stained and a fluorescent image of the tissue slice in which a specific biological substance is stained with a fluorescent staining reagent (S10), extracts a cell nucleus from the image of the cell nucleus (S20), extracts a fluorescent bright point from the fluorescent image (S30), specifies a cell nucleus to which the fluorescent bright point is assigned on the basis of the distance between the cell nucleus and the fluorescent bright point, and assigns the fluorescent bright point with the cell nucleus (S40).

主权项

1. An image processing device comprising:
an input unit to input a bright field image of a tissue slice in which a cell nucleus is stained and a fluorescent image of the tissue slice in which a specific biological substance is stained with a fluorescent staining reagent; a first extracting unit to extract a cell nucleus from the bright field image; a second extracting unit to extract a fluorescent bright point from the fluorescent image; an assigning unit to specify a cell nucleus to which the fluorescent bright point is assigned on the basis of a distance between the cell nucleus and the fluorescent bright point and to assign. the fluorescent bright point to the cell nucleus; and an excluding unit to exclude a fluorescent bright point after assignment processing from the assigned fluorescent bright points on the basis of an average distance between a surface of the cell nucleus and the fluorescent bright point after assignment processing, inclination or angle between straight lines connecting the fluorescent bright points after assignment processing, or the N/C ratio of the cell to which the fluorescent bright point is assigned.