发明名称 |
Intermittent detection during analytical reactions |
摘要 |
Methods, devices, and systems for performing intermittent detection during analytical reactions are provided. Such methods facilitate collection of reaction data from disparate reaction times. Further, such methods are useful for reducing photo-induced damage of one or more reactants in an illuminated analytical reaction at a given reaction time. In preferred embodiments, the reaction mixture is subjected to at least one illuminated and non-illuminated period and allowed to proceed such that the time in which the reaction mixture is illuminated is less than a photo-induced damage threshold period. |
申请公布号 |
US9556480(B2) |
申请公布日期 |
2017.01.31 |
申请号 |
US201514708603 |
申请日期 |
2015.05.11 |
申请人 |
Pacific Biosciences of California, Inc. |
发明人 |
Turner Stephen;Sorenson Jon;Maxham Kenneth Mark;Eid John |
分类号 |
C12Q1/68;C12N9/12;G01N21/64;G06F19/22 |
主分类号 |
C12Q1/68 |
代理机构 |
|
代理人 |
Scherer David C.;Arnold Deana A. |
主权项 |
1. A method of performing a nucleic acid sequencing reaction, comprising:
a) preparing a reaction mixture comprising components of the nucleic acid sequencing reaction including a polynucleotide to be sequenced; b) initiating the nucleic acid sequencing reaction in the reaction mixture to begin progression of the nucleic acid sequencing reaction, wherein the nucleic acid sequencing reaction is initiated during a non-illuminated period, and wherein the reaction mixture comprises an enzyme selected from the group consisting of: a polymerase, a ligase, a ribosome, a nuclease, and a kinase; and c) maintaining conditions that allow the nucleic acid sequencing reaction to proceed while subjecting the reaction mixture to at least one illuminated period following the non-illuminated period, wherein illumination data is collected during the at least one illuminated period, thereby performing the nucleic acid sequencing reaction. |
地址 |
Menlo Park CA US |