Non-recombinant <i>saccharomyces </i>strains that grow on xylose

摘要

The present invention relates to methods for producing Saccharomyces strains that are capable of growth on xylose as a sole carbon source at a desired growth rate, (such as at least one generation per 48 hours), strains made by such methods, and Saccharomyces strains that grow at a growth rate of at least one generation per 48 hours using xylose as a sole carbon source for growth made by non-recombinant methods.

主权项

1. A non-recombinant Saccharomyces cerevisiae strain that produces an increase in biomass in the range of 2-fold to 49-fold when grown in 50 ml xylose minimal medium shaken at 220 rpm in a 250 ml Erlenmeyer flask at 30° C. for 48 hours, wherein the strain is inoculated into the xylose minimal media to an OD600nm of 0.1 to 0.2 after being grown for 72 hours at 30° C. on a Glucose Yeast Extract Bacteriological Peptone agar plate, and wherein the xylose minimal media is 5% xylose and 0.67% DIFCO Yeast Nitrogen base without amino acids in distilled water.