| 摘要 |
The invention relates to a process for making an electrode modified with a nanostructured assembly of gold and L-cysteine nanoparticles used for electrochemically determining and studying athenolol. According to the invention, the process consists in grinding, with alumina, a vitreous coal electrode, the ultrasonic treatment thereof with ethanol and deionized water, the electro-polymerization, by cyclic voltammetry of the glutamic acid, to obtain a compact layer of polyglutamic acid, the carboxyl groups of the polyglutamic acid are activated by the immersion into a solution of 10 mM 1-ethyl-3-carbodiimide for 20 minutes, the electrode is immersed into a solution of 10 mM of N-hydroxysuccinimide for 20 minutes, at the surface of the polyglutamic acid there are bonded the molecules of the L-cysteine by using the reaction (EDC/NHS), the groups -SH at the surface of the electrode are bonded with the Au nanoparticles having the diameter of 40 nm, which were previously obtained by reducing HAuClwith sodium citrate, the electrode, thus obtained, having the measurement range between 10...10M, the detection limit of 3.9x10M, with a good sensitivity of 5.09x10mA/decade and reproducibility. |
