发明名称 |
SEQUENCE-INDEPENDENT NUCLEIC ACID ASSEMBLY |
摘要 |
Disclosed is a method for assembling nucleic acid fragments in a sequence-independent way. The method entails attaching a nucleic acid fragment to a solid phase, and contacting the first nucleic acid fragment with a second nucleic acid fragment such that the second nucleic acid fragment is ligated to the first nucleic acid fragment in a directional way. These steps can be repeated multiple times to assemble additional nucleic acid fragments. The method can further include a step of recovering the ligated nucleic acid to obtain the assembled nucleic acid. |
申请公布号 |
WO2017015348(A1) |
申请公布日期 |
2017.01.26 |
申请号 |
WO2016US43104 |
申请日期 |
2016.07.20 |
申请人 |
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA |
发明人 |
COLEMAN, Todd, Prentice;KYRIAKAKIS, Phillip |
分类号 |
C40B50/14;C07K1/00;C12Q1/68;C40B50/18 |
主分类号 |
C40B50/14 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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