METHODS AND KITS TO IDENTIFY KLEBSIELLA STRAINS

摘要

Embodiments of the invention provide a method of detecting one or more strains of Klebsiella pneumoniae. The method may include forming a plurality of mixtures for nucleic amplification. The method can include amplification of specific sequences within the K. pneumonia genome that can provide definitive information to distinguish between one or more types or strains of K. pneumonia.

主权项

1. A method of detecting carbapenem-resistant Enterobacteriaceae in a sample, the method comprising the steps of:
adding a first, a second, and a third oligonucleotide to a mixture comprising the sample, wherein the first, second, and third oligonucleotides added to the mixture include a sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, and SEQ ID NO: 4; subjecting the mixture to conditions that allow nucleic acid amplification; and detecting the carbapenem-resistant Enterobacteriaceae in the sample on the basis of results of the nucleic acid amplification in the mixture.