| 发明名称 |
SYNTHETIC MRNA LEADERS |
| 摘要 |
The present invention provides a synthetic mRNA leader for enhancing the expression of a gene encoding a desired protein, vectors comprising said synthetic mRNA leader and methods of producing a desired gene product using said synthetic mRNA leader and vector, said method comprising expressing said gene using a synthetic mRNA leader which comprises from 5′ to 3′: (i) a first mRNA leader sequence element; (ii) a spacer region; and (iii) a second mRNA leader sequence element; wherein said first mRNA leader sequence element is a modified transcription-stimulating mRNA leader capable of enhancing transcription of a gene relative to an unmodified reference mRNA leader sequence and/or said second mRNA leader element is a modified translation-stimulating mRNA leader capable of enhancing the translation of a gene transcript relative to an unmodified reference mRNA leader sequence. |
| 申请公布号 |
US2017022509(A1) |
申请公布日期 |
2017.01.26 |
| 申请号 |
US201515300993 |
申请日期 |
2015.04.02 |
| 申请人 |
VECTRON BIOSOLUTIONS AS |
发明人 |
LALE Rahmi;VALLA Svein;BALZER LE Simone |
| 分类号 |
C12N15/67;C12N15/70;C12N15/78;C12Q1/68 |
主分类号 |
C12N15/67 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method of enhancing expression of a desired gene product in a recombinant gene expression system, said method comprising expressing said gene using a synthetic mRNA leader which comprises from 5′ to 3′:
(i) a first mRNA leader sequence element; (ii) a spacer region; and (iii) a second mRNA leader sequence element;
wherein said first mRNA leader sequence element is a modified transcription-stimulating mRNA leader capable of enhancing transcription of a gene relative to an unmodified reference mRNA leader sequence and/or said second mRNA leader element is a modified translation-stimulating mRNA leader capable of enhancing the translation of a gene transcript relative to an unmodified reference mRNA leader sequence. |
| 地址 |
Trondheim NO |
