METHOD FOR GENERATING ENDOTHELIAL COLONY FORMING CELL-LIKE CELLS

摘要

The present disclosure relates generally to methods and compositions useful in cell and tissue biology and therapeutics. In particular, an in vitro method for differentiating pluripotent cells into endothelial colony forming cell-like cells (ECFC-like cells) is provided. A purified human cell population of NRP-1+CD31+ ECFC-like cells is provided, wherein at least some of the cells in the population have a high proliferation potential. Therapeutic and test agent screening methods for using the cell populations of the present disclosure are provided.

主权项

1. A method for generating an isolated population of human endothelial colony forming cell-like cells (ECFC-like cells) from human pluripotent stem cells, the method comprising:
a) providing pluripotent stem cells; b) inducing the pluripotent stem cells to undergo endothelial differentiation, wherein inducing comprises: i) culturing the pluripotent stem cells for about 24 hours in an endothelial differentiation medium comprising Activin A, BMP-4, VEGF and FGF-2; and ii) replacing the medium of step i) with an endothelial differentiation medium comprising BMP-4, VEGF and FGF-2 about every one or two days thereafter; and c) isolating from the cells induced to undergo differentiation the ECFC-like cells, wherein the ECFC-like cells are CD31+NRP-1+ and exhibit a cobblestone morphology.