| 主权项 |
1. A method of concurrently detecting a first analyte of interest and a second analyte of interest comprising:
contacting a solid support with a first analyte of interest, a second analyte of interest, a bridge unit comprising a second capture reagent, and a signal detection unit comprising a third capture reagent; and detecting the presence or absence of the signal detection unit which indicates the presence or absence of the first analyte of interest and second analyte of interest concurrently, wherein: a first capture reagent is affixed to the solid support; the first analyte of interest comprises a first interaction unit that binds to the first capture reagent and a second interaction unit that binds to the bridge unit; and the second analyte of interest comprises a first interaction unit and a second interaction unit, wherein the first interaction unit binds the bridge unit; a signal detection unit that binds to: i) the second analyte, ii) to the second analyte's first interaction unit or second interaction unit, iii) to a component of the first and second analyte complex, or iv) a component of an analyte-bridge complex that is only present when the complex contains the first and second analytes. |
