CONTROLLING TRANSLOCATION THROUGH NANOPORES WITH FLUID WALLS

摘要

Improved resolution and detection of nanoparticles are achieved when a nanopore connecting liquid compartments in a device running on the Coulter principle is provided with fluid coatings such as lipid walls. Fluid lipid walls are made of a lipid bilayer, and preferably include lipid anchored mobile ligands as part of the lipid bilayer. By varying the nature and concentration of the mobile ligand in the lipid bilayer, multifunctional coatings of lipids are provided.

主权项

1. A method of detecting, quantifying, or characterizing a biomolecule, collection of biomolecules, or particles, comprising:
providing a transit path for biomolecules or particles to pass through a nanopore from a first liquid compartment to a second liquid compartment, wherein the first and second liquid compartments contain electrodes disposed to measure voltage difference, current flow, or resistance between the first and second liquid compartments; and measuring voltage difference, current flow, or resistance between the two liquid compartments over time as individual biomolecules pass through the nanopore; wherein the nanopore is a passageway through a substrate, which passageway is lined with a fluid wall.