| 主权项 |
1. A bioreactor system comprising:
a) one vessel assembly comprising at least one closed tubular rigid culture vessel; wherein said culture vessel comprises at least one tube and two closed ends made of single use or reusable material; wherein said culture vessel is positioned to lie along the longitudinal axis; wherein said culture vessel is configured to culture cells; wherein said culture vessel comprises at least one baffle; wherein the baffle contacts the inside surface and two closed ends of said culture vessel, wherein the baffle has a ratio of greater than 0.1 and less than 0.9 of heights of said baffle to the cross-sectional surface of said culture vessel perpendicular to the longitudinal axis; wherein said culture vessel comprises at least one media port configured to allow culture material to flow in and out of said culture vessel; and wherein said culture vessel comprises at least one set of two gas ports configured to allow the respiratory gas to flow in and out of said culture vessel; b) one reservoir assembly comprising at least one closed container made of single use or reusable material; c) one rocking apparatus configured to hold and position said culture vessel and rock about the longitudinal axis of the culture vessel along the plane perpendicular to said axis at angle of less than 360 degrees; d) one pumping apparatus fluidly coupled to at least one media port of said culture vessel and configured to pump culture material in and out of said culture vessel through said at least one port; e) one gassing apparatus coupled to at least one set of two gas ports of said culture vessel and configured to entering the gas through one gas port and out of said culture vessel through another gas port; f) one gas blending apparatus coupled to said gassing apparatus and configure to blend the gases including oxygen, nitrogen, carbon dioxide, or air; g) one monitoring apparatus configured to monitor one or more parameters of said vessel assembly including culture material of said culture vessel, reservoir assembly, pumping apparatus, gassing apparatus, gas blending apparatus, and rocking apparatus; and h) one control apparatus connected wired or wirelessly to vessel assembly, reservoir assembly, pumping apparatus, gassing apparatus, gas blending apparatus, rocking apparatus and monitoring apparatus wherein said control apparatus is configured and programmed to coordinate the positioning and movement of said culture vessel using said rocking apparatus with the culture material pumping in and out of said culture vessel using said pumping apparatus, the passing respiratory gases to enter and exit said culture vessel through one set of inlet/outlet ports and remaining in the gas space at all times using the gassing apparatus; to adjust the gas concentration using said gas blending apparatus and to control the operating parameters of said vessel assembly monitored by said monitoring apparatus. |
