发明名称 |
METHODS FOR MONITORING CELLULAR STATES AND FOR IMMORTALIZING MESENCHYMAL STEM CELL |
摘要 |
We describe a method of monitoring the state of a cell, the method comprising establishing, for a selected microRNA (miRNA) species secreted by the cell, a ratio of: (a) a precursor form of the miRNA species (pre-miRNA); to (b) a mature form of the miRNA species (mature miRNA); in which the pre- to mature miRNA ratio so established is indicative of the state of the cell. We also describe a method comprising the steps of: (a) providing a mesenchymal stem cell (MSC); and (b) introducing an oncogene into the mesenchymal stem cell to thereby transform it; in which the transformed mesenchymal stem cell does not secrete a gene product of the oncogene into a medium in which it is grown. |
申请公布号 |
US2017020925(A1) |
申请公布日期 |
2017.01.26 |
申请号 |
US201615188794 |
申请日期 |
2016.06.21 |
申请人 |
Agency for Science, Technology and Research |
发明人 |
Lim Sai Kiang |
分类号 |
A61K35/28;C12N5/0775;C12Q1/68 |
主分类号 |
A61K35/28 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
Singapore SG |