| 摘要 |
In some embodiments, the present invention a mutated FAD-GDHa protein, wherein the mutated FAD-GDHa protein is mutated from a wild-type first species to contain at least one point mutation, wherein the mutated FAD-GDHα protein comprises: P(X)n=8X4(X)n=16V(X)n=6RN(X)n=3YDXRPXCXGX3NNCMP(X)n=1CP(X)n=2A(X)n=1Y(X)n=1G(X)n=6A(X)n=2AG(X)n=6AVV(X)n=3E(X)n=8-9A(X)n=2Y(X)n=1D(X)n=5HRV(X)n=5V(X)n=2A(X)n=3E(X)n=2K(X)n=4S(X)n=5P(X)n=1G(X)n=2N(X)n=4GRN(X)n=1MDH(X)n=4V(X)n=1F(X)n=6-7W(X)n=1GRGP(X)n=9RDGXX5R(X)n=19T(X)n=14L(X)n=14X2(X)n=1X1(X)n=1E(X)n=4P(X)n=1NR(X)n=3S(X)n=4D(X)n=2G(X)n=7Y(X)n=4Y(X)n=32-35, wherein each X represents a wild-type amino acid residue of the first species and n indicates the number of the wild-type amino acid residues of the first species represented by a respective parenthetical at that position, wherein: a) X1 is selected from the group consisting of X, S, C, T, M, V, Y, N, P, L, G, Q, A, I, D, W, H, and E, wherein if X1 is L, H or V, then X2 is D; b) X3 is selected from the group consisting of G, H, D, Y, S, and X; c) X4 is selected from the group consisting of S and X; and d) X5 is selected from the group consisting of S and X. |
