发明名称 |
ANTIBODIES AND METHODS FOR THE DETECTION OF CELL DEATH |
摘要 |
Disclosed herein are antibodies having binding specificity to the amino acid sequences Ala Ser Ser Gly Leu Thr Val Glu Val Asp (SEQ ID NO:1 ) and Thr Val Glu Val Asp (SEQ ID NO:14), and methods of detecting cell death in a sample, comprising contacting the sample with a first antibody specific for a C-terminal amino acid sequence Ala Ser Ser Gly Leu Thr Val Glu Val Asp (SEQ ID NO:1 ) or Thr Val Glu Val Asp (SEQ ID NO:14) of a CK18 protein fragment having a C-terminal amino acid sequence of Val Glu Val Asp (SEQ ID NO:2) and a second antibody that specifically binds an epitope that is present in both full-length CK18 and the CK18 protein fragment, and that does not overlap with SEQ ID NO:1 or SEQ ID NO:14, under conditions such that the CK1 8 protein fragment present in the sample specifically binds to the first antibody and the second antibody, wherein one of the antibodies is bound to a solid support and the other antibody is bound to a detection moiety capable of producing a signal; optionally removing any unbound or excess material; and detecting the signal from the detection moiety, wherein the signal is positively correlated with the presence of the CK18 protein fragment in the sample. |
申请公布号 |
EP3119805(A1) |
申请公布日期 |
2017.01.25 |
申请号 |
EP20150764318 |
申请日期 |
2015.03.20 |
申请人 |
Wellstat Diagnostics, LLC |
发明人 |
MILLER, Jeffrey, Allan;JEFFRIES, Eddie, Phillip |
分类号 |
C07K16/18;G01N33/53;G01N33/58 |
主分类号 |
C07K16/18 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|