Structuring fats and methods of producing structuring fats

摘要

Recombinant DNA techniques are used to produce oleaginous recombinant cells that produce triglyceride oils having desired fatty acid profiles and regiospecific or stereospecific profiles. Genes manipulated include those encoding stearoyl-ACP desturase, delta 12 fatty acid desaturase, acyl-ACP thioesterase, ketoacyl-ACP synthase, and lysophosphatidic acid acyltransferase. The oil produced can have enhanced oxidative or thermal stability, or can be useful as a frying oil, shortening, roll-in shortening, tempering fat, cocoa butter replacement, as a lubricant, or as a feedstock for various chemical processes. The fatty acid profile can be enriched in midchain profiles or the oil can be enriched in triglycerides of the saturated-unsaturated-saturated type.

主权项

1. A recombinant cell of the genus Prototheca comprising at least 20% lipid by dry cell weight, wherein the cell is optionally obligately heterotrophic, the cell comprising one or more exogenous nucleic acids that encode an acyl transferase, a thioesterase, a ketoacyl ACP synthase (KAS), a ketoacyl CoA synthase (KCS), or an elongase, wherein the fatty acid profile of the cell is altered by the expression of the one or more exogenous nucleic acids and the lipid is in enriched in stearate-oleate-stearate (SOS) triacylglycerides as compared to non-recombinant cell of the genus Prototheca.