| 发明名称 |
Recombinant host cells comprising phosphoketolases |
| 摘要 |
The present invention is related to recombinant host cells comprising: (i) at least one deletion, mutation, and/or substitution in an endogenous gene encoding a polypeptide that converts pyruvate to acetaldehyde, acetyl-phosphate, or acetyl-CoA; and (ii) a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity. The present invention is also related to recombinant host cells further comprising (iii) a heterologous polynucleotide encoding a polypeptide having phosphotransacetylase activity. |
| 申请公布号 |
US9550999(B2) |
申请公布日期 |
2017.01.24 |
| 申请号 |
US201414335734 |
申请日期 |
2014.07.18 |
| 申请人 |
Butamax Advanced Biofuels LLC |
发明人 |
Dauner Michael;Maggio-Hall Lori Ann;Tomb Jean-Francois |
| 分类号 |
C12N1/20;C12N1/00;C12N9/00;C12N15/00;C12P7/16;C07H21/04;C12N15/81;C12N9/02;C12N9/10;C12N9/88;C12N15/52;C12N15/74;C12P7/18;C12P7/26;C12P7/46;C12P7/56;C12P13/06;C12P13/08 |
主分类号 |
C12N1/20 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A recombinant host cell comprising:
(i) at least one genetic modification in an endogenous gene encoding a polypeptide having pyruvate decarboxylase activity, wherein the at least one genetic modification reduces or eliminates pyruvate decarboxylase activity; and (ii) a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity. |
| 地址 |
Wilmington DE US |
