Cloning and exploitation of a functional R-gene from <i>Solanum chacoense</i>

摘要

The invention relates to a resistance gene and functional homologues or fragments thereof isolated from S. chacoense, S. berthaultii, S. sucrense or S. tarijense. More over, the invention relates to the use of said resistance gene, for example the use of said resistance gene in a method to increase or confer at least partial resistance in a plant to an oomycete infection. The invention provides an isolated or recombinant nucleic acid sequence comprising a nucleic acid sequence encoding one of the amino acid sequences of FIG 4 or a functional fragment or a functional homologue thereof such as those presented in FIG. 13.

主权项

1. A method for increasing resistance in a Solanaceae plant against infection by Phytophthora infestans, said method comprising introducing into said plant or a part thereof a nucleic acid that expresses a nucleotide sequence encoding a protein of the amino acid sequence SEQ ID NO:126 or a homolog thereof;
wherein the homolog is a protein having an amino acid sequence selected from the group consisting of SEQ ID NO:194; SEQ ID NO:198; SEQ ID NO:203; SEQ ID NO:205; SEQ ID NO:207; SEQ ID NO:209; SEQ ID NO:211; SEQ ID NO:217; SEQ ID NO:219; SEQ ID NO:221; and SEQ ID NO:223; and which homolog confers resistance to P. infestans in Solanaceaein a detached leaf assay; wherein said plant exhibits increased resistance to said infection as compared to a plant into which said nucleic acid has not been introduced.