Rational design of microRNA-siRNA chimeras for multi-functional target suppression

摘要

The present disclosure encompasses methods for rational design of microRNA and small interfering RNA chimeras and compositions and methods of use thereof.

主权项

1. A composition, the composition comprising a nucleic acid construct comprising a nucleotide sequence of about 19 to about 25 nucleotides in which nucleotides 2 through 8 are identical to nucleotides 2 through 8 of an miRNA and the rest of the nucleotides are identical to those of a siRNA that specifically hybridizes to a target nucleic acid, wherein nucleotides 2 through 8 are capable of specifically hybridizing to nucleic acids that are naturally regulated by the miRNA and the entire nucleic acid construct is capable of specifically hybridizing to the target nucleic acid targeted by the siRNA.