Production of Lentiviral Vectors

摘要

A high-volume gene therapy vector manufacturing process which produces a recombinant gene therapy vector which is able to transform host cells even when they are not dividing.

主权项

1. A method comprising:
a. Transducing a mammalian cell with a baculovirus to make a transduced mammalian producer cell; and then b. Culturing said transduced mammalian producer cell in culture media, and harvesting from said transduced mammalian producer cell and/or culture media a second virus having a therapeutic transgene.