| 主权项 |
1. A method of selecting a target double-stranded polynucleotide molecule, said target have an internal destabilized region, wherein the destabilized region comprises (i) an intact polynucleotide strand in the target double-stranded polynucleotide and (ii) a strand substantially complementary to the intact strand and with a plurality of abasic sites or mismatches, said method comprising the steps of:
(a) contacting the internal destabilized region with a single-stranded polynucleotide, said single-stranded polynucleotide comprising a linker portion and a capture portion, said linker portion having a sequence sufficiently complementary to hybridize to the intact polynucleotide strand of the destabilized region of the target under appropriate conditions, and said capture portion does not hybridize to the destabilized region of the target; and (b) contacting the target with a capture substance that interacts with the capture portion of the single-stranded polynucleotide to select the target. |
