| 摘要 |
According to the present invention, viable cells of microorganisms in a test sample are detected and distinguished from dead cells and/or damaged cells using the steps below: a) a step of adding, to the test sample, an agent that selectively inhibits, in dead cells, the amplification of nucleic acids of the microorganisms by means of a nucleic acid amplification method, b) a step of carrying out processing to increase the permeability of the cells of the microorganisms, c) a step of amplifying, by means of an isothermal nucleic acid amplification method that uses a strand-displacement nucleic acid elongase, nucleic acid target regions of the microorganisms in the test sample without extracting nucleic acids from the cells, and d) a step of analyzing the amplification product. |
