| 主权项 |
1. A method of collecting a fluid sample from a test subject with an automated fluid sampling device, the method comprising:
connecting the test subject to the automated fluid sampling device, the fluid sampling device including:
a pump being moveable in a first direction to draw fluid into the pump and a second direction to expel fluid from the pump;a reservoir in fluid communication with the pump, the reservoir having a first opening and a second opening;a first conduit fluidly connecting the reservoir to a first fluid fitting configured to fluidly connect the reservoir to tubing having a distal end inserted into the test subject;a second conduit fluidly connecting the reservoir to a second fluid fitting configured to connect the reservoir to a sample collection component; anda third conduit fluidly connecting the reservoir to a third fluid fitting configured to connect the reservoir to a sterile fluid supply;wherein the pump is configured to move in the first direction and second direction in response to movement of a portion of the fluid sampling device and the conduits are configured such that a first valve of the fluid sampling device controls fluid flow in the first conduit, a second valve of the fluid sampling device controls fluid flow in the second conduit, and a third valve of the fluid sampling device controls fluid flow in the third conduit, each valve having an open state and a closed state; and wherein the fluid sampling device includes a controller for controlling the pump and valve; activating a predetermined routine on the automated fluid sampling device, said predetermined routine including the steps of:
opening the first valve, and moving the pump in the first direction to draw sample fluid through the first conduit and into the reservoir; thereby forming a sample fluid/sterile fluid interface;opening the second valve, and moving the pump in the second direction to force a sample fluid from the reservoir through the second conduit to the sample collection component, the sample collection component collecting a predetermined amount of fluid;re-opening the first valve, and moving the pump in the second direction to force the sterile fluid out of the first fluid fitting, thereby flushing the first conduit;re-opening the second valve, and moving the pump in the second direction to force the sterile fluid out of the second fluid fitting, thereby flushing the second conduit and the sample collection component, andopening the third valve, and moving the pump in the first direction to draw the sterile fluid through the third fluid fitting and into the pump through the first opening of the third conduit; wherein said activating step is performed by depressing a single button on the automated fluid sampling device. |
