发明名称 |
Compositions and methods for producing plants resistant to glyphosate herbicide |
摘要 |
The present disclosure includes the production of a mutant plant resistant to an herbicide of the phosphonomethylglycine family, e.g. glyphosate. Compositions and methods are provided for editing a nucleotide sequence of interest in a cell employing a guide polynucleotide/Cas endonuclease system, wherein the Cas endonuclease is guided by a guide polynucleotide to recognize and optionally introduce a double strand break at a specific target site into the genome of a cell. The nucleotide sequence of interest to be edited can be located within or outside the target site that is recognized by a Cas endonuclease. More specifically, compositions and methods are provided for editing an enolpyruvylshikimate-3-phosphate synthase (EPSPS) nucleotide sequence in a cell. The methods and compositions employ a guide polynucleotide/Cas endonuclease system to provide for an effective system for editing EPSPS nucleotide sequences of within the genome of a cell. Also provided are compositions and methods for the production of glyphosate tolerant plant cells, plants explants, seeds and grain. |
申请公布号 |
AU2015288157(A1) |
申请公布日期 |
2017.01.19 |
申请号 |
AU20150288157 |
申请日期 |
2015.07.01 |
申请人 |
Pioneer Hi-Bred International, Inc.;E. I. du Pont de Nemours and Company |
发明人 |
Djukanovic, Vesna;Jones, Spencer Charles;Liu, Zhan-Bin;Lassner, Michael;Lyznik, L. Aleksander |
分类号 |
A01H1/02;C12N15/113;C12N15/82 |
主分类号 |
A01H1/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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