| 摘要 |
The present invention relates to the field of molecular pathology (for example cancer diagnosis, prognosis, treatment and/or therapy prediction) through the detection of RNA, mutations, copy number changes and determination of the methylation status of specific sequences of the genome of individual patients in hybridization assays (southern blot, ISH, dot blot) including in situ determination of the methylation status of specific sequences of the genome of individual patients in individual cells. More specifically the present invention relates to: a) target-specific probes covalently-attached to a labeled tail, b) the synthesis method of said probe, c) the usage of said probe such as an in situ hybridization-based method to correlate the methylation status of a promoter region of a gene in a biopsy or cytology specimen of a patient to the morphology and localization in that specimen, and d) kits comprising said target-specific probes. The latter method and products allow detection of (epi) genetic changes in specific cell types of histological or cytological (cancer) specimens or on membranes which will contribute to scientific research and which will help physicians to accurately diagnose diseases and/or start an appropriate treatment. |
