Distribution of refractive index measurement by synthetic aperture tomography

摘要

The present invention relates to systems and methods for quantitative three-dimensional mapping of refractive index in living or non-living cells, tissues, or organisms using a phase-shifting laser interferometric microscope with variable illumination angle. A preferred embodiment provides tomographic imaging of cells and multicellular organisms, and time-dependent changes in cell structure and the quantitative characterization of specimen-induced aberrations in high-resolution microscopy with multiple applications in tissue light scattering.

主权项

1. A synthetic aperture method for measuring refractive index of a medium comprising:
transmitting a focused beam of light along a first light path through a medium; combining the light transmitted through the medium with a reference light; detecting the combined light; determining a phase image and an amplitude image of the medium; combining the phase and amplitude images to form an electrical field image of the medium; and determining the distribution of a refractive index of the medium using synthetic aperture analysis.