Methods and devices related to a regulated flow electroporation chamber

摘要

The electroporation chamber and its related devices combine the features of an electroporation chamber that acts as a manifold for regulation of sample flow with those of a flow electroporation device to form a regulated flow electroporation device. The invention further comprises a novel regulated flow electroporation chamber that enables conditions in which a sample is uniformly processed in individual fractions or volumes in a fully closed (sterile) system.

主权项

1. A flow electroporation chamber comprising:
(a) at least two electrodes separated by a spacer, the electrodes and the spacer configured to form a single, continuous chamber within the spacer; and (b) at least three ports configured to traverse the spacer to communicate through the chamber within the spacer, wherein
(i) a first port is for sample flow into the chamber;(ii) a second port is for processed sample flow out of the chamber; and(iii) a third port is for non-sample fluid or gas flow in or out of the chamber, wherein the non-sample fluid or gas flows out of the chamber when a sample flows into the chamber and the non-sample fluid or gas flows into the chamber when processed sample flows out of the chamber.