DYRKNINGSSUBSTRAT TIL HUMANE PLURIPOTENTE STAMCELLER OG ANVENDELSE DERAF

摘要

The present invention provides a culture substrate which enables maintenance culture of human pluripotent stem cells in a pluripotent state under a feeder-free culture environment, and a culture method of human pluripotent stem cells using the culture substrate. By seeding human pluripotent stem cells dissociated into single cells at a cell density of 4×10 4 to 10×10 4 cells/cm 2 onto a culture substrate coated with human laminin ±5²1³1 E8 fragment or human laminin ±3²3³2 E8 fragment preferably at a concentration of 0.5 to 25 µg/cm 2 , the human pluripotent stem cells can be rapidly expanded in a pluripotent state.