| 主权项 |
1. A system for imaging biological samples disposed in culture media, the system comprising:
a system calibration module that provides default values for capturing an image of a biological sample disposed on culture media disposed in a plate; an image acquisition module comprising a camera wherein the module is adapted to acquire data for a series of images at a given time interval, in communication with the system calibration module, the image acquisition configured to: i) acquire data for a first image using default values for photon flux and exposure time from the system calibration module and creating a pixel by pixel map of the image data, each pixel associated with a signal to noise ratio, a photon flux and exposure time, and an intensity; ii) update the image acquisition time by reviewing the image data to identify saturated pixels and selecting one of a new photon flux, a new exposure time or both based on whether the number of saturated pixels is greater than or less than a predetermined threshold and, based on that determination; iii) use the new value for the photon flux, the exposure time or both to acquire data for a new image and iv) update the map of the image data with the new values for signal to noise ratio, the photon flux, the exposure time or both and pixel intensity, wherein the image acquisition module is configured to acquire data for a new image if the signal to noise ratio of unsaturated pixels is less than a predetermined threshold or the number of saturated pixels exceeds the predetermined threshold, wherein the image acquisition module finalizes image acquisition when either the predetermined threshold signal to noise ratio has been obtained for the non-saturated pixels, the predetermined allocated time for image acquisition has elapsed or the predetermined maximum number of images has been acquired; and an image presentation module that transforms the image data from the image acquisition module into an image for viewing or analysis. |
