Method for Enhancing N-acetylglucosamine Production through glcK Knockout of Bacillus subtilis

摘要

The present invention provides a method for enhancing N-acetylglucosamine production by usage of a recombinant Bacillus subtilis with a glcK knockout. This invention enhanced the production of GlcNAc by knocking out the glcK gene which encodes a glucokinase, thus eliminating the GlcNAc phosphorylation to GlcNAc-6-P. The specific growth rate and content of GlcNAc in the supernatant of the recombinant Bacillus subtilis with the glcK knockout were 0.15 h−1 and 3.0 g/L, respectively, which were 2.32 times and 2.14 times of those of the control strain without glcK knockout. The recombinant Bacillus subtilis of the present invention would be potentially useful for industrial production of GlcNAc.

主权项

1. A method for enhancing N-acetylglucosamine (GlcNAc) production of Bacillus subtilis, comprising:
a), knocking out a glcK gene of the strain BSGN6-PxylA-glmS-P43-GNA1 to obtain a recombinant glcK knockout Bacillus subtilis; and b), using the recombinant glcK knockout Bacillus subtilis obtained in step a) to produce GlcNAc, wherein said glcK gene encodes a glucokinase; and wherein said BSGN6-PxylA-glmS-P43-GNA1 is constructed from Bacillus subtilis 168 Δ nagP Δ gamP Δ gamA Δ nagA Δ nagB Δ ldh Δ pta::lox72, and the expression of glmS and GNA1 in said BSGN6-PxylA-glmS-P43-GNA1 are under the control of promoter PxylA and P43, respectively.